Guide 5' tGAGGTAGTagGTTGTATAgt 3'
Target 3' ugCUCCAUCAcuCAACAUAU 5'
Figure 1. Sequence of the guide strand and target strand crystallized with Argonaute (3F73). Bases that base-pair are uipper-case, and non-pairing bases are lower-case.
|
T. thermophilus |
Arg |
Lys |
Gln |
Ile |
Leu |
Asn |
Arg |
Asn |
Gly |
Lys |
Ala |
|
A. aeolicus |
Tyr |
Lys |
Gln |
Val |
Ile |
Leu |
Val |
Asn |
Gln |
Lys |
Ser |
|
P. furiosus |
Phe |
Lys |
Gln |
Val |
Val |
Asn |
Val |
Asn |
Gln |
Lys |
Gly |
Figure 2. Anchoring site residues in three species. Conserved residues are in bold.