Image 1: The 3rd step of glycolysis requires the enzyme as its committed step to metabolizing a glucose into a pyruvate.
Source: Top: Reference 9 Bottom: ChemBioDraw
Image 2: Four different binding pockets for the substrate fructose-6-phosphate near the N-terminal halves of ScPFK.
Source: Reference 10 and Paint
Image 3: The pockets where fructose-2,6-bisphosphate bind near the C-terminal halves of ScPFK.
Source: Reference 10 and Paint
Image 4: Rotation between active (R State) and inactive (T State) PFK.
Source: Reference 14